gram method

Gram's method

[gramz]
noun (sometimes lowercase)
a method of staining and distinguishing bacteria, in which a fixed bacterial smear is stained with crystal violet, treated with Gram's solution, decolorized with alcohol, counterstained with safranine, and washed with water.


Origin:
named after Hans C. J. Gram (1853–1938), Danish bacteriologist

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Gram's method
 
n
bacteriol Gram-negative See also Gram-positive a staining technique used to classify bacteria, based on their ability to retain or lose a violet colour, produced by crystal violet and iodine, after treatment with a decolorizing agent
 
[C19: named after Hans Christian Joachim Gram (1853--1938), Danish physician]

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Medical Dictionary

Gram's method (grāmz)
n.
A staining technique used to classify bacteria in which a bacterial specimen is first stained with crystal violet, then treated with an iodine solution, decolorized with alcohol, and counterstained with safranine. Gram-positive bacteria retain the violet stain; gram-negative bacteria do not. Also called Gram's stain.

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