Gram's method

noun (sometimes lowercase)
a method of staining and distinguishing bacteria, in which a fixed bacterial smear is stained with crystal violet, treated with Gram's solution, decolorized with alcohol, counterstained with safranine, and washed with water.

named after Hans C. J. Gram (1853–1938), Danish bacteriologist Unabridged
Based on the Random House Dictionary, © Random House, Inc. 2014.
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World English Dictionary
Gram's method
bacteriol Gram-negative See also Gram-positive a staining technique used to classify bacteria, based on their ability to retain or lose a violet colour, produced by crystal violet and iodine, after treatment with a decolorizing agent
[C19: named after Hans Christian Joachim Gram (1853--1938), Danish physician]

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American Heritage
Medical Dictionary

Gram's method (grāmz)
A staining technique used to classify bacteria in which a bacterial specimen is first stained with crystal violet, then treated with an iodine solution, decolorized with alcohol, and counterstained with safranine. Gram-positive bacteria retain the violet stain; gram-negative bacteria do not. Also called Gram's stain.

The American Heritage® Stedman's Medical Dictionary
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