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grams method

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Gram's method

[gramz]
–noun (sometimes lowercase)
a method of staining and distinguishing bacteria, in which a fixed bacterial smear is stained with crystal violet, treated with Gram's solution, decolorized with alcohol, counterstained with safranine, and washed with water.


Origin:
named after Hans C. J. Gram (1853–1938), Danish bacteriologist
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Based on the Random House Dictionary, © Random House, Inc. 2009.
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Medical Dictionary

Gram's method (grāmz)
n.
A staining technique used to classify bacteria in which a bacterial specimen is first stained with crystal violet, then treated with an iodine solution, decolorized with alcohol, and counterstained with safranine. Gram-positive bacteria retain the violet stain; gram-negative bacteria do not. Also called Gram's stain.

The American Heritage® Stedman's Medical Dictionary
Copyright © 2002, 2001, 1995 by Houghton Mifflin Company. Published by Houghton Mifflin Company.
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