Now, char a portion of the sample, dissolve in water and pour into the separatory funnel, letting it run slowly into the flask.
Cool and filter into a separatory funnel, washing with cold water.
Pour the filtrate into a separatory funnel, add an equal volume of ether and two volumes of water.
Wash the combined extracts in a separatory funnel three times with 25-cc.
Extract with five times its volume of ether by agitation in a separatory funnel.
Therefore, wherever we find peculiar effects of that separatory operation, we have a right to infer the proper cause.
Disinfect the filter candle and separatory funnel by boiling.
Draw off the petroleum ether layer as closely as possible by means of a slender glass siphon into a separatory funnel of 500 cc.
Attach the nozzle of the separatory funnel to the filter candle by means of the perforated rubber stopper (Fig. 37).