Tire out, exhaust, as in Running errands all day did me in. [Colloquial; early 1900s] Also see done in.
Kill, as in Mystery writers are always thinking of new ways to do their characters in. [Slang; early 1900s] Also see def. 4.
Ruin utterly; also cheat or swindle. For example, The five-alarm fire did in the whole block, or His so-called friend really did him in. [First half of 1900s]
do oneself in. Commit suicide, as in She was always threatening to do herself in. [Slang; first half of 1900s]