By repeating this process two or three times, the fluorescein may be obtained in a very pure condition.
If we fill a glass container with a fluorescein solution and look at it by reflected light it appears green.
fluorescein is readily nitrated, yielding a di- or tetra-nitro compound according to conditions.
fluorescein fluo·res·ce·in (flu-rěs'ē-ĭn, flô-)
An orange-red compound that exhibits intense fluorescence in alkaline solution and is used in ophthalmology to reveal corneal lesions.